Development and characterization of 25 microsatellite primers for Ilex chinensis (Aquifoliaceae)1
نویسندگان
چکیده
PREMISE OF THE STUDY To evaluate genetic variation and structure of Ilex chinensis (Aquifoliaceae), a dioecious evergreen tree, we developed 25 microsatellite markers from its nuclear genome. METHODS AND RESULTS Based on the biotin-streptavidin capture method, 10 polymorphic and 15 monomorphic microsatellite markers were developed. Ten polymorphic loci were characterized by 87 individuals sampled from three populations located in Zhejiang Province and Shanghai, China. The number of alleles per locus varied from two to 12. The observed and expected heterozygosities were 0.0435-0.9032 and 0.3121-0.8343, respectively. CONCLUSIONS These microsatellite markers can be useful for further genetic studies of I. chinensis populations, and so contribute to forest restoration and management.
منابع مشابه
Shotgun sequencing for microsatellite identification in Ilex paraguariensis (Aquifoliaceae)1
UNLABELLED PREMISE OF THE STUDY Ilex paraguariensis is a native tree species from Brazil, Argentina, and Paraguay that is used in the production of beverages, medicines, and cosmetics. Primers flanking microsatellites were developed to investigate genetic parameters in the species. • METHODS AND RESULTS Using microsatellites cloned from an I. paraguariensis shotgun genomic library, 25 pair...
متن کاملDevelopment of microsatellite markers in Ilex kaushue (Aquifoliaceae), a medicinal plant species1
PREMISE OF THE STUDY Microsatellite markers were developed for Ilex kaushue (Aquifoliaceae), a medicinal plant with extremely small wild populations that exists in fragmented habitats, to assess and protect its genetic diversity. METHODS AND RESULTS Using 454 GS FLX Titanium sequencing, 16 microsatellite primer sets were isolated and characterized. Fifteen of these markers were polymorphic. T...
متن کاملTwo New Triterpenoid Glycosides from Leaves of Hex chinensis SIMS1)
Two new triterpenoid glycosides were isolated (one of them as the methyl ester) as major components from leaves of Ilex chinensis Sims (Aquifoliaceae) . The structures of these glycosides, termed ilexoside A (1) and ilexoside B methyl ester (2a), have been established to be 3ƒÀ-ƒ-(ƒÀ-Dxylopyranosyl)siaresinolic acid and 3ƒÀ-ƒ-(ƒÀ-D-xylopyranosyl)pomolic acid methyl ester , respectively, based o...
متن کاملMicrosatellite Isolation and Characterization in Pomegranate (Punica granatum L.)
Development of microsatellite markers has been an increasing trend in crop genetic studies because oftheir applicability in breeding programs. Here we report the development of inter simple sequencerepeat (SSRs) in pomegranate (Punica granatum L.) using an enrichment method that makes use of magneticbeads. Enriched genomic libraries with AG and ATG microsatellite motifs were c...
متن کاملIsolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii)
Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 pri...
متن کامل